生态毒理学进入 MudPIT 时代？

Ecotoxicology goes MudPIT?

机构信息

Pathology Department, Stavanger University Hospital, Armauer Hansens Road 20, Stavanger, Norway.

出版信息

Mar Environ Res. 2010;69 Suppl:S34-6. doi: 10.1016/j.marenvres.2010.03.008. Epub 2010 Mar 23.

DOI:10.1016/j.marenvres.2010.03.008

PMID:20403633

Abstract

Exposure to natural and anthropogenic compounds can potentially alter the proteome in body fluids and tissues of living organisms, and by applying proteomics it is possible to discover, identify and understand such alterations. This study show results from a proteomic approach where one- or multidimensional separation (MudPIT) combined with high-accuracy tandem mass spectrometry (i.e. LTQ Orbitrap) were used to identify proteins from a non-model organism (Salmo salar). An optimized two-dimensional method resulted in more than 680 proteins identified with high significance compared to 197 proteins identified using a one-dimensional separation. Thus, MudPIT proteomics greatly increase the number of successful protein identification studies in ecotoxicology, and could potentially provide more insight into chemical modes of actions.

摘要

暴露于自然和人为化合物可能会改变生物体体液和组织中的蛋白质组，通过应用蛋白质组学可以发现、识别和理解这种改变。本研究展示了一种蛋白质组学方法的结果，其中一维或多维分离（MudPIT）与高精度串联质谱（即 LTQ Orbitrap）相结合，用于鉴定非模式生物（大西洋鲑）的蛋白质。优化的二维方法鉴定了超过 680 种具有高显著性的蛋白质，而一维分离方法仅鉴定了 197 种蛋白质。因此，MudPIT 蛋白质组学极大地增加了生态毒理学中成功的蛋白质鉴定研究的数量，并可能为化学作用模式提供更多的见解。