Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, PR China.
Bioresour Technol. 2010 Sep;101(18):7005-10. doi: 10.1016/j.biortech.2010.03.132. Epub 2010 Apr 18.
To the aim of efficient utilization of both of xylose and cellulose, a laboratory xylose/cellulose fractionation and separate fermentation (XCFSF) bioethanol process was performed. Three xylose/cellulose fractionation strategies: (A) dilute sulfur acid hydrolysis and detoxification, (B) lime pretreatment and xylanase hydrolysis, (C) bio-treatment with Phanerochaete chrysosporium and xylanase hydrolysis were applied to corn cobs. As a result, the maximum xylose yields obtained from A, B and C fractionation methods were 78.47%, 57.84% and 42.54%, respectively, and 96.81%, 92.14% and 80.34% of cellulose were preserved in the corresponding solid residues. The xylose dissolved in acid and enzymatic hydrolysates was fermented to ethanol by Candida shahatae and the cellulose remaining in solid residues was converted to ethanol by simultaneous saccharification and fermentation (SSF) with Saccharomyces cerevisiae. Finally, for A, B, C fractionation methods, 70.40%, 52.87%, 39.22% of hemicellulose and 89.77%, 84.30%, 71.90% of cellulose in corn cobs was converted to ethanol, respectively.
为了有效利用木糖和纤维素，采用了实验室木糖/纤维素分级分离发酵（XCFSF）生物乙醇工艺。采用了三种木糖/纤维素分级分离策略：（A）稀硫酸水解和脱毒，（B）石灰预处理和木聚糖酶水解，（C）黄孢原毛平革菌生物处理和木聚糖酶水解，分别对玉米芯进行处理。结果表明，A、B 和 C 分级方法的最大木糖得率分别为 78.47%、57.84%和 42.54%，相应的固体残渣中分别保留了 96.81%、92.14%和 80.34%的纤维素。酸和酶解液中溶解的木糖被 Candida shahatae 发酵成乙醇，固体残渣中剩余的纤维素通过同时糖化和发酵（SSF）用 Saccharomyces cerevisiae 转化为乙醇。最后，对于 A、B、C 分级方法，玉米芯中的半纤维素分别转化为乙醇的比例为 70.40%、52.87%、39.22%，纤维素的转化率分别为 89.77%、84.30%、71.90%。
