鼠伤寒沙门氏菌高亲和力甲硫氨酸转运基因的克隆

Cloning of high-affinity methionine transport genes from Salmonella typhimurium.

作者信息

Shaw N A, Ayling P D

机构信息

Department of Applied Biology, University of Hull, U.K.

出版信息

FEMS Microbiol Lett. 1991 Mar 1;62(2-3):127-31. doi: 10.1016/0378-1097(91)90145-z.

DOI:10.1016/0378-1097(91)90145-z

PMID:2040419

Abstract

At least one of the genes encoding a methionine transport system in Salmonella typhimurium has been cloned on a 15 kbp fragment in the lambda 1059 vector, and the region responsible for the transport activity was subcloned in a set of plasmids with inserts ranging from 1.4 to 4.2 kbp in the multicopy plasmid pUC8. Two proteins of Mr 34 and 40 kDa were expressed from the larger inserts.

摘要

鼠伤寒沙门氏菌中编码甲硫氨酸转运系统的至少一个基因已克隆到λ1059载体的一个15千碱基对片段上，负责转运活性的区域被亚克隆到一组质粒中，这些质粒的插入片段在多拷贝质粒pUC8中介于1.4至4.2千碱基对之间。从较大的插入片段中表达出了分子量为34千道尔顿和40千道尔顿的两种蛋白质。