Key Laboratory of Molecular Biology for Infectious Diseases of the State Ministry of Education, Institute for Viral Hepatitis, The Second Affiliated Hospital, Chongqing Medical University, 74# Linjiang Road, 400010, Chongqing, People's Republic of China.
Med Oncol. 2011 Sep;28(3):804-9. doi: 10.1007/s12032-010-9520-5. Epub 2010 Apr 20.
Dicer, the key enzyme in the RNAi pathway, is misregulated in tumor tissues. The altered expression of Dicer is associated with clinical characteristics in patients with cancer. Liver carcinoma and adjacent non-neoplastic tissues were obtained from 36 patients with hepatocellular carcinoma (HCC) undergoing surgery. Expressions of Dicer mRNA were evaluated using the Real-time reverse transcription-PCR in 36 liver carcinoma tissues and 36 adjacent histologically non-cancerous liver tissues. Dicer mRNA levels were evaluated in relation to age, sex, tumor number, tumor size, tumor stage, and distant metastasis. Dicer mRNA level was significantly lower in malignant tissues than in the corresponding non-neoplastic tissues in 34 of the 36 patients with HCC (94.4%). The Dicer expression level was not associated with clinical characteristics, including age, sex, tumor number, tumor size, tumor stage, or distant metastasis in HCC cases. These results demonstrate that Dicer is significantly down-regulated in HCC, suggesting that reduced expression of Dicer may play an important role during the process of hepatocarcinogenesis.
Dicer，RNAi 通路中的关键酶，在肿瘤组织中失调。Dicer 的异常表达与癌症患者的临床特征有关。从 36 名接受手术的肝细胞癌 (HCC) 患者中获得肝癌和相邻非肿瘤组织。使用 Real-time RT-PCR 评估 36 个肝癌组织和 36 个相邻组织学上无癌性肝组织中的 Dicer mRNA 表达。评估了 Dicer mRNA 水平与年龄、性别、肿瘤数量、肿瘤大小、肿瘤分期和远处转移的关系。在 36 例 HCC 患者中，34 例恶性组织中的 Dicer mRNA 水平明显低于相应的非肿瘤组织 (94.4%)。在 HCC 病例中，Dicer 表达水平与临床特征（包括年龄、性别、肿瘤数量、肿瘤大小、肿瘤分期或远处转移）无关。这些结果表明 Dicer 在 HCC 中显著下调，提示 Dicer 表达降低可能在肝癌发生过程中发挥重要作用。
