2nd Department of Internal Medicine, Cell Analysis Laboratory, Semmelweis University, Szentkiralyi Street 46, 1088 Budapest, Hungary.
Pathol Oncol Res. 2011 Mar;17(1):11-6. doi: 10.1007/s12253-010-9262-x. Epub 2010 Apr 21.
Bone marrow derived mesenchymal stem cells (BM-MSCs) take part in the colonic mucosal regeneration. They are multipotent cells, which can be identified with both negative (i.e. CD13, CD 14, CD45, c-Kit, major histocompatibility complex /MHC class I and II) and positive (i.e. CD54 (ICAM1), CD133, CD146 (MCAM), CD166, Flk-1, Sca-1, Thy-1, stage-specific antigen I /SSEA-I and Musashi-1, HLA class I) markers. These cells can repopulate the gastrointestinal mucosa as they may differentiate into stromal- (i.e. myofi-broblast) or epithelial-like (Paneth-, epithel-, goblet or enteroendocrin) cells without proliferation. During the mesenchymal to epithelial transition (MET) stem cells enter the epithelial layer and take up epithelial cell-like properties. Rarely BM-MSCs may retain their stem cell characteristics and are capable of producing progeny. The isolated lymphoid aggregates may serve as a platform from where BM-MSCs migrate to the nearby crypts as mediated by several chemoattractant proteins, which are expressed in injured tissue. The number of BM-MSCs is influenced by the degree of inflammation. In this review we summarize the current information about the role of BM-MSCs in the repair progress of injured colonic epithelium and their potential clinical applications.
骨髓间充质干细胞（BM-MSCs）参与结肠黏膜的再生。它们是多能细胞，可以通过阴性（即 CD13、CD14、CD45、c-Kit、主要组织相容性复合体/MHC Ⅰ类和Ⅱ类）和阳性（即 CD54（ICAM1）、CD133、CD146（MCAM）、CD166、Flk-1、Sca-1、Thy-1、阶段特异性抗原 I/SSEA-I 和 Musashi-1、HLA Ⅰ类）标志物来识别。这些细胞可以重新填充胃肠道黏膜，因为它们可以分化为基质（即肌成纤维细胞）或上皮样（潘氏、上皮、杯状或肠内分泌）细胞而不增殖。在间充质向上皮转化（MET）过程中，干细胞进入上皮层并获得上皮细胞样特性。罕见情况下，BM-MSCs 可能保留其干细胞特性并能够产生后代。分离的淋巴聚集物可以作为一个平台，BM-MSCs 通过几种趋化因子蛋白从中迁移到附近的隐窝，这些蛋白在受损组织中表达。BM-MSCs 的数量受炎症程度的影响。在这篇综述中，我们总结了关于 BM-MSCs 在受损结肠上皮修复过程中的作用及其潜在临床应用的最新信息。
