沙利度胺和己酮可可碱对实验性自身免疫性脑脊髓炎（EAE）的影响。
Effect of thalidomide and pentoxifylline on experimental autoimmune encephalomyelitis (EAE).
机构信息
Fluminense Federal University, FCB, Nova Friburgo, Rio de Janeiro, Brazil.
出版信息
Exp Neurol. 2010 Nov;226(1):15-23. doi: 10.1016/j.expneurol.2010.04.007. Epub 2010 Apr 18.
BACKGROUND
Autoimmune encephalomyelitis (EAE) in Lewis rats is a classical experimental model of demyelinating inflammatory disease of the central nervous system. EAE is widely accepted for study of immune-inflammatory mechanisms in the CNS related to multiple sclerosis (MS) due to similar clinical evolution.
OBJECTIVES
In the present study we investigated the effects of Thalidomide and pentoxifylline during EAE development in Lewis rats.
METHODS
EAE was induced in Lewis rats and treatment with Thalidomide or pentoxifylline was performed. Clinical evaluation was carried out daily. Histopathological analysis of the brain tissue and spinal cord was performed. Griess method was used for determination of NO serum levels. TNF-alpha and IFN-gamma serum levels were investigated using ELISA method.
RESULTS
Thalidomide and pentoxifylline treatment is associated with significant reduction of neuroinflammation in CNS. Serum levels of NO, IFN-gamma and TNF-alpha showed a marked reduction. Such findings were correlated with improvement of clinical symptoms, particularly in thalidomide treated rats.
CONCLUSIONS
Taken together the data suggested that thalidomide and pentoxifylline may be therapeutic options for the treatment of MS, however further experiments must be performed to investigate this hypothesis.
背景
实验性自身免疫性脑脊髓炎（EAE）在 Lewis 大鼠中是一种中枢神经系统脱髓鞘炎症性疾病的经典动物模型。由于与多发性硬化症（MS）相关的中枢免疫炎症机制相似，EAE 被广泛用于 MS 的研究。
目的
本研究旨在探讨沙利度胺和己酮可可碱在 Lewis 大鼠 EAE 发病过程中的作用。
方法
诱导 Lewis 大鼠 EAE，并给予沙利度胺或己酮可可碱治疗。每日进行临床评估。对脑组织和脊髓组织进行组织病理学分析。采用 Griess 法测定血清中 NO 的水平，采用 ELISA 法测定血清中 TNF-α和 IFN-γ的水平。
结果
沙利度胺和己酮可可碱治疗与 CNS 中神经炎症的显著减轻有关。血清中 NO、IFN-γ和 TNF-α的水平明显降低。这些发现与临床症状的改善相关，尤其是在沙利度胺治疗的大鼠中。
结论
综合数据表明，沙利度胺和己酮可可碱可能是治疗 MS 的治疗选择，但必须进一步实验来验证这一假设。
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