Heterogeneity of calbindin-containing neurons in the mouse main olfactory bulb: I. General description.

The structural features of calbindin-positive neurons were studied in the mouse main olfactory bulb (MOB). Calbindin-positive neurons were heterogeneous, including numerous periglomerular cells, a few granule cells, small to medium-sized interneurons in the external plexiform layer, and large short-axon cells located in the external plexiform layer, internal plexiform layer, granule cell layer and ependymal/subependymal layer. These large short-axon cells were also heterogeneous; some corresponded to classically identified short-axon cells such as Blanes cells, Golgi cells, horizontal cells and vertical cells, but some others appeared to be previously unidentified. A few faintly calbindin-positive presumed tufted cells were also encountered. Near the ependymal/subependymal layer of the MOB some calbindin-positive short-axon cells extended their dendritic processes more or less parallel to the sagittal plane, presumably corresponding to medullary cells named recently. In addition we encountered a few calbindin-positive horizontal cells in the internal plexiform layer extending their axons toward the lateral olfactory tract, one of which was confirmed to extend its axon into the lateral olfactory tract, indicating that they were presumed to be one of projection neurons. The present study revealed the diversity of calbindin-positive neurons in the mouse MOB and their particular structural properties hitherto unknown.