通过酶联免疫吸附测定（ELISA）快速检测抗原特异性小鼠IgE。
Rapid detection of antigen-specific mouse IgE by enzyme-linked immunosorbent assay (ELISA).
作者信息
Strouse R J, Anderson D W, Argentieri D C
机构信息
R.W. Johnson Pharmaceutical Research Institute, Department of Immunopharmacology Raritan, New Jersey 08869.
出版信息
J Immunoassay. 1991;12(1):113-24. doi: 10.1080/01971529108055060.
A rapid, sensitive, antigen-specific mouse IgE capture ELISA is described. A monoclonal rat anti-mouse IgE was used as the capture antibody, and a DNP-coupled BSA-biotinylated conjugate along with a peroxidase-avidin-biotin complex was utilized as the detection system. The lower detection limit of this assay is 8.5 ng/ml of antigen-specific IgE. With some modifications, this assay can be employed to screen for antigen specific antibodies of other isotypes and subtypes.
本文描述了一种快速、灵敏、抗原特异性的小鼠IgE捕获ELISA。使用单克隆大鼠抗小鼠IgE作为捕获抗体，并将DNP偶联的BSA-生物素化缀合物与过氧化物酶-抗生物素蛋白-生物素复合物一起用作检测系统。该检测方法的最低检测限为8.5 ng/ml抗原特异性IgE。经过一些修改，该检测方法可用于筛选其他同种型和亚型的抗原特异性抗体。
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