Gomathi K G, Sharma E, Sharma S K
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi.
J Immunol Methods. 1991 May 17;139(1):101-5. doi: 10.1016/0022-1759(91)90356-k.
C6 glioma cell conditioned medium (C6CM) has been used as a growth supplement for murine hybridomas. At 10% C6CM has been found to increase cell proliferation by 3-4-fold. This effect is observed in the presence of saturating concentration of FCS. Clonal growth is also enhanced 6.7-fold. No growth promoting effect is seen on murine myelomas or spleen cells. The factor(s) appear to be protein in nature.
C6胶质瘤细胞条件培养基（C6CM）已被用作小鼠杂交瘤的生长补充剂。已发现10%的C6CM可使细胞增殖增加3至4倍。在存在饱和浓度胎牛血清（FCS）的情况下可观察到这种效应。克隆生长也增强了6.7倍。对小鼠骨髓瘤或脾细胞未见生长促进作用。这些因子似乎本质上是蛋白质。
