成像表面等离子体共振在原位研究海洋生物表面探索中的新应用。

Novel application of imaging surface plasmon resonance for in situ studies of the surface exploration of marine organisms.

机构信息

Department of Physics, Chemistry and Biology, Division of Molecular Physics, Linkoping University, Linkoping SE-581 83, Sweden.

出版信息

Biointerphases. 2009 Dec;4(4):65-8. doi: 10.1116/1.3274060.

DOI:10.1116/1.3274060

PMID:20408726

Abstract

The surface interactions of exploring cyprids of the barnacle Semibalanus balanoides were studied in situ using imaging surface plasmon resonance. It was demonstrated how the deposition of a proteinaceous adhesive could be followed in real time as the cyprids explored and temporarily attached to a surface. Furthermore, the amount of protein left on the surface when the cyprids moved on could be quantified. Clear differences were demonstrated between an oligo(ethyleneglycol) coated surface and a bare gold substrate. It is anticipated that this technique will be a valuable tool in the development of novel surface chemistries that can prevent biofouling.

摘要

使用成像表面等离子体共振原位研究了藤壶幼体探索过程中的表面相互作用。实验演示了如何实时跟踪蛋白质粘性物质的沉积过程，当藤壶幼体探索并暂时附着在表面上时，该过程就会发生。此外，还可以定量分析藤壶幼体移动时留在表面上的蛋白质量。实验结果表明，与涂有寡聚乙二醇的表面相比，裸金基底存在明显差异。预计该技术将成为开发可防止生物污垢的新型表面化学物质的有价值工具。