Institute of Materials Research and Engineering, (A*STAR) Agency for Science, Technology and Research, Singapore, Singapore.
Biofouling. 2009 Oct;25(7):611-9. doi: 10.1080/08927010903033621.
The behavioural response of cypris larvae from A. amphitrite (=Balanus amphitrite) exploring three model glass surfaces is quantified by close-range microscopy. Step length and step duration measurements reveal a response to both surface properties and flow. Without flow, 2-day-old cyprids took larger steps with shorter step duration on hydrophilic glass surfaces (bare and NH2-treated) vs hydrophobic glass (CH3-treated). These parameters suggest a more detailed, local inspection of hydrophobic surfaces and a more extensive exploration for hydrophilic surfaces. Cyprids under flow took longer steps and exhibited shorter probing times on hydrophobic glass. On hydrophilic glass, cyprids increased their step duration under flow. This active response is attributed to drag and lift forces challenging the cyprids' temporary anchoring to the substratum. Seven-day-old cyprids showed almost no discrimination between the model surfaces. Microscopic-scale observation of cyprid exploration is expected to provide new insights into interactions between cyprids and surfaces.
利用近景显微镜对十足幼虫（即藤壶）探索三种模型玻璃表面的行为反应进行定量分析。步长和步幅的测量结果表明，幼虫对表面特性和水流均有反应。在没有水流的情况下，2 日龄的幼虫在亲水玻璃表面（裸玻璃和 NH2 处理过的玻璃）上的步幅更大，步幅持续时间更短，而在疏水玻璃表面（CH3 处理过的玻璃）上的步幅则更小。这些参数表明，幼虫对疏水表面进行了更详细、更局部的检查，对亲水表面进行了更广泛的探索。在水流作用下，幼虫在疏水玻璃上的步幅更长，探测时间更短。而在亲水玻璃上，幼虫在水流作用下增加了步幅持续时间。这种主动反应归因于水流的拖曳力和升力，这些力挑战了幼虫暂时附着在基底上的能力。7 日龄的幼虫几乎无法区分模型表面。对幼虫探索的微观尺度观察有望为幼虫与表面之间的相互作用提供新的见解。
