Microbial Pathogenicity Laboratory, Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, India.
J Appl Microbiol. 2010 Sep;109(3):946-52. doi: 10.1111/j.1365-2672.2010.04721.x.
To evaluate whole cell protein profiling vis-à-vis genotyping to discern phylogenetic relationships among strains of Y. enterocolitica biovar 1A.
Whole cell protein profiling of Y. enterocolitica biovar 1A strains was performed using SDS-PAGE. Twenty-one distinct protein profile types were obtained among a collection of 81 strains isolated from clinical and nonclinical sources. Whole cell protein profiling exhibited discriminatory index (DI) of 0·80 and clustered the strains into two distinct clonal groups. The clinical and the aquatic serotype O:6,30-6,31 strains were clustered into two discrete subgroups.
Whole cell protein profiling displayed sufficient diversity among strains of Y. enterocolitica biovar 1A, and the phylogenetic relationships obtained were in good agreement with those established earlier by genotyping techniques.
Whole cell protein profiling was as discriminatory as some of the genotyping methods and has the potentiality to be used as an adjunct tool to study epidemiology of Y. enterocolitica.
通过全细胞蛋白分析与基因分型比较，以辨别 1A 生物型小肠结肠炎耶尔森菌菌株的系统发育关系。
采用 SDS-PAGE 对 1A 生物型小肠结肠炎耶尔森菌进行全细胞蛋白分析。从临床和非临床来源分离的 81 株菌中获得了 21 种不同的蛋白图谱类型。全细胞蛋白分析显示分辨指数（DI）为 0.80，并将菌株聚类为两个不同的克隆群。临床和水生血清型 O:6,30-6,31 菌株聚类为两个不同的亚群。
1A 生物型小肠结肠炎耶尔森菌的全细胞蛋白分析显示出菌株间具有足够的多样性，所得的系统发育关系与先前通过基因分型技术建立的关系非常吻合。
全细胞蛋白分析与一些基因分型方法一样具有分辨能力，有可能作为研究小肠结肠炎耶尔森菌流行病学的辅助工具。
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