Departments of Allergy and Immunology, Gunma Children's Medical Center, 779 Shimohakoda, Hokkitsu-machi, Shibukawa, Gunma, Japan.
Pediatr Allergy Immunol. 2011 Feb;22(1 Pt 2):e87-94. doi: 10.1111/j.1399-3038.2010.01026.x.
Because little information is available on eosinophil activation and cytokine response in virus-induced wheezing, we attempted to detect respiratory viruses and measure eosinophil cationic protein (ECP), and 27 types of cytokines/chemokines in both serum and nasal secretions from children with wheezing. This study was an observational, case-control investigation of 267 subjects, who were visited and/or hospitalized with acute respiratory symptoms (with wheezing: men, 115; women, 59; mean/median age, 3.6/3.0 years) or who were visited for regular physical examination and treatment (non-symptomatic wheezing: men, 48; women, 31; mean/median, 5.0/4.7 years), and 14 control subjects (controls: men, 9; women, 5; mean/median, 3.6/3.7 years). We detected viruses in nasal secretions from 174 patients with acute exacerbations of wheezing using antigen detection kits or reverse transcription-polymerase chain reaction, followed by direct DNA sequencing analysis. We measured peripheral eosinophil counts, and serum concentrations of ECP and 27 cytokines/chemokines using a multiplex bead-based assay in patients with wheezing or non-symptomatic wheezing. We also examined nasal ECP and 27 cytokines/chemokines in patients with wheezing. Of 174 samples from wheezing exacerbations, rhinovirus was detected in 59; respiratory syncytial (RS) virus in 44; enterovirus in 17; other viruses in 19; and no viruses in 35. Serum concentrations of ECP, IL-5, IL-6, IL-1ra, and IP-10 were significantly elevated in rhinovirus-induced wheezing compared with non-symptomatic wheezing. Similarly, serum ECP, IL-5, and IP-10 were significantly higher in rhinovirus-induced wheezing than in controls. On the other hand, IL-1ra and IP-10, but not ECP and IL-5 were significantly higher in RS virus-induced wheezing than in controls. Furthermore, only IL-5 was significantly elevated in the rhinovirus group compared with the RS virus group in both serum and nasal secretions. Different cytokine profile and eosinophil activation might be involved in rhinovirus- and RS virus-induced acute exacerbation of childhood wheezing.
由于关于病毒诱导性喘息中嗜酸性粒细胞激活和细胞因子反应的信息较少，我们试图检测呼吸道病毒并测量喘息儿童血清和鼻分泌物中的嗜酸性粒细胞阳离子蛋白（ECP）和 27 种细胞因子/趋化因子。这项研究是一项观察性的病例对照研究，共纳入 267 名受试者，其中包括因急性呼吸道症状就诊和/或住院（喘息：男性 115 例，女性 59 例，平均/中位数年龄 3.6/3.0 岁）或因常规体检和治疗就诊的非喘息儿童（非喘息性喘息：男性 48 例，女性 31 例，平均/中位数年龄 5.0/4.7 岁），以及 14 名对照者（对照组：男性 9 例，女性 5 例，平均/中位数年龄 3.6/3.7 岁）。我们使用抗原检测试剂盒或逆转录聚合酶链反应检测了 174 名急性喘息加重患儿的鼻分泌物中的病毒，然后进行直接 DNA 测序分析。我们使用基于微珠的多重分析检测了喘息和非喘息儿童的外周血嗜酸性粒细胞计数以及血清 ECP 和 27 种细胞因子/趋化因子的浓度。我们还检测了喘息儿童的鼻 ECP 和 27 种细胞因子/趋化因子。在 174 例喘息加重的样本中，59 例检测到鼻病毒，44 例检测到呼吸道合胞病毒（RSV），17 例检测到肠道病毒，19 例检测到其他病毒，35 例未检测到病毒。与非喘息性喘息相比，鼻病毒诱导的喘息患者血清 ECP、IL-5、IL-6、IL-1ra 和 IP-10 的浓度显著升高。同样，鼻病毒诱导的喘息患者血清 ECP、IL-5 和 IP-10 也显著高于对照组。另一方面，与对照组相比，RS 病毒诱导的喘息患者血清 IL-1ra 和 IP-10 升高，但 ECP 和 IL-5 没有升高。此外，在血清和鼻分泌物中，与 RSV 组相比，只有 IL-5 在鼻病毒组中显著升高。病毒诱导的儿童喘息急性加重可能涉及不同的细胞因子谱和嗜酸性粒细胞激活。
