Department of Marine Biology, College of Marine Life Sciences, Ocean University of China, Qingdao, China.
J Fish Biol. 2010 Apr;76(5):1149-58. doi: 10.1111/j.1095-8649.2010.02562.x.
A novel cell line (bmGH) was established from the heart of brown-marbled grouper Epinephelus fuscoguttatus and its viral susceptibility was evaluated. The bmGH cells have been subcultured to passage 65 in Dulbecco's modified eagle medium:Ham's nutrient mixture F-12 (1:1) medium (DMEM/F12) which was further supplemented with foetal bovine serum (FBS), carboxymethyl-chitosan, basic fibroblast growth factor (bFGF) and insulin-like growth factor-I (IGF-I) at 24 degrees C. The heart cells have a fibroblastic morphology and proliferated to confluence 14 days later. The cells grew at a steady rate during subsequent subculture and had a population doubling time of 40.3 h at passage 60. Karyotype analysis showed that these cells exhibited chromosomal aneuploidy with a modal chromosome number of 48. The results of viral susceptibility characterization revealed that cytopathic effects (CPE) of bmGH cells appeared after infection by two iridoviruses, turbot reddish body iridovirus (TRBIV) and lymphocystis disease virus (LCDV). A large number of TRBIV and LCDV particles were also observed in the infected bmGH cells by electron microscope examination. All of these facts indicate that the bmGH cell line established here may serve as a valuable tool for studies of cell-virus interactions and has potential applications in fish virus isolation, propagation and vaccine development.
从褐点石斑鱼（Epinephelus fuscoguttatus）心脏建立了一种新型细胞系（bmGH），并评估了其病毒易感性。bmGH 细胞已在 DMEM/F12 培养基（DMEM/F12）中传代至第 65 代，该培养基进一步补充了胎牛血清（FBS）、羧甲基壳聚糖、碱性成纤维细胞生长因子（bFGF）和胰岛素样生长因子-I（IGF-I），在 24°C 下培养。心细胞呈成纤维细胞形态，14 天后增殖至汇合。随后的传代中，细胞以稳定的速度生长，在第 60 代时群体倍增时间为 40.3 小时。核型分析显示这些细胞表现出染色体非整倍性，模式染色体数为 48。病毒易感性特征分析结果表明，两种虹彩病毒（TRBIV 和 LCDV）感染 bmGH 细胞后出现细胞病变效应（CPE）。电子显微镜检查还观察到大量的 TRBIV 和 LCDV 颗粒存在于感染的 bmGH 细胞中。所有这些事实表明，这里建立的 bmGH 细胞系可作为研究细胞-病毒相互作用的有用工具，并具有在鱼类病毒分离、增殖和疫苗开发中的潜在应用。
