形态学精子缺陷的分析通过光学显微镜和透射电子显微镜及其与精子运动的相关性。

Morphological sperm defects analyzed by light microscopy and transmission electron microscopy and their correlation with sperm motility.

机构信息

Department of Experimental Medicine, Pasteur Institute - Cenci Bolognetti Foundation, University of Rome Sapienza, Rome, Italy.

出版信息

Int J Urol. 2010 Mar;17(3):259-66. doi: 10.1111/j.1442-2042.2010.02451.x.

DOI:10.1111/j.1442-2042.2010.02451.x

PMID:20409218

Abstract

OBJECTIVES

To compare sperm defects as assessed by light microscopy (LM) and transmission electron microscopy (TEM), and to correlate them with sperm motility.

METHODS

A cohort of 40 male partners of infertile couples was selected. Group 1 (n = 31) included subjects with motility >5 and <50%, group 2 (n = 9) included those with motility <5% and the control group consisted of 10 normospermic subjects. Semen analysis of morphological parameters was carried out by LM and TEM.

RESULTS

A linear correlation between LM and TEM regarding head defects and excess residual cytoplasm (r = 0.87 and 0.90) was found, whereas there was a poor correlation between tail and midpiece anomalies (r = 0.46 and 0.21). No significant variations were detected by LM and TEM regarding sperm head defects and excess residual cytoplasm, whereas TEM showed a significantly greater percentage of tail and midpiece alterations compared with LM in groups 1 and 2, as well as controls (P < 0.05). The microtubular pattern '<9 + 2' represented the most frequent axonemal morphological alteration.

CONCLUSIONS

TEM might represent an additional diagnostic tool in the presence of severe sperm hypomotility or absence of motility.

摘要

目的

比较通过光镜（LM）和透射电镜（TEM）评估的精子缺陷，并将其与精子活力相关联。

方法

选择了 40 名不育夫妇的男性伴侣进行研究。第 1 组（n = 31）包括活力> 5%且<50%的受试者，第 2 组（n = 9）包括活力<5%的受试者，对照组由 10 名正常精子的受试者组成。通过 LM 和 TEM 对精子形态学参数进行分析。

结果

发现 LM 和 TEM 在头部缺陷和过多残留细胞质方面呈线性相关（r = 0.87 和 0.90），而尾部和中段异常之间的相关性较差（r = 0.46 和 0.21）。LM 和 TEM 在精子头部缺陷和过多残留细胞质方面没有发现显著差异，但在第 1 组和第 2 组以及对照组中，TEM 显示出比 LM 更高的尾部和中段改变的百分比（P < 0.05）。“<9 + 2”微管模式代表最常见的轴丝形态改变。