Soulez B, Dei-Cas E, Palluault F, Camus D
I.N.S.E.R.M. (U. 42), Villeneuve d'Ascq, France.
J Parasitol. 1991 Jun;77(3):449-53.
Changes in Pneumocystis carinii induced by the extraction of the parasite from rabbit lung have been investigated. Samples obtained using 4 extraction methods were evaluated by light and transmission electron microscopy. Light microscopic evaluation was insufficient to give a measure of the P. carinii viability or to detect parasitic cellular alterations. In contrast, ultrastructural evaluation provided information on host and P. carinii cell integrity, which is a critical condition for viability. None of the tested methods was ideal. How thoroughly and in what shape P. carinii need to be extracted from tissues will determine which extraction technique is of best use.
已对从兔肺中提取卡氏肺孢子虫所引起的变化进行了研究。使用4种提取方法获得的样本通过光学显微镜和透射电子显微镜进行评估。光学显微镜评估不足以衡量卡氏肺孢子虫的活力或检测寄生虫细胞的改变。相比之下，超微结构评估提供了关于宿主和卡氏肺孢子虫细胞完整性的信息，这是生存能力的关键条件。所测试的方法均不理想。从组织中彻底提取卡氏肺孢子虫的程度和形态将决定哪种提取技术最适用。
