Hiszczyńska-Sawicka Elzbieta, Akhtar Masood, Kay Graham William, Holec-Gasior Lucyna, Bickerstaffe Roy, Kur Józef, Stankiewicz Mirosław
Faculty of Agriculture and Life Sciences, Lincoln University, PO Box 84, Lincoln 7647, Canterbury, New Zealand.
Vet Immunol Immunopathol. 2010 Aug 15;136(3-4):324-9. doi: 10.1016/j.vetimm.2010.03.018. Epub 2010 Mar 30.
The aim of this study is to compare the immune responses of sheep stimulated by the intramuscular injection of a liposome formulated-DNA plasmid encoding the Toxoplasma gondii MAG1 antigen only or co-expressed with ovine IL-6. Forty-five, 2-year-old sheep were divided into four groups. Group 1 received an empty pVAXIg plasmid, group 2 no treatment, group 3 liposome formulated plasmid pVAXIgMAG1 and group 4, pVAXIgMAG1 plus pVAXovIL-6 plasmids. All the animals were inoculated at weeks 0 and 4. The injection of sheep with a plasmid encoding for MAG1 only or a MAG1 plasmid co-expressed with a plasmid encoding for ovine IL-6 produced humoral immune responses. The plasmids containing MAG1 elevated significantly serum IgG1 and IgG2 levels 2 weeks and onwards after the first injection of the plasmids. Co-expression of IL-6 with MAG1 had no effect on IG1 or IG2 levels illustrating that IL-6 in the formulation used had no modulating effect on any measured immune response.
本研究的目的是比较仅通过肌肉注射编码刚地弓形虫MAG1抗原的脂质体配方DNA质粒或与绵羊IL-6共表达所刺激的绵羊免疫反应。将45只2岁绵羊分为四组。第1组接受空的pVAXIg质粒，第2组不进行处理，第3组为脂质体配方质粒pVAXIgMAG1，第4组为pVAXIgMAG1加pVAXovIL-6质粒。所有动物在第0周和第4周接种。给绵羊注射仅编码MAG1的质粒或与编码绵羊IL-6的质粒共表达的MAG1质粒可产生体液免疫反应。含有MAG1的质粒在首次注射质粒后2周及以后显著提高血清IgG1和IgG2水平。IL-6与MAG1的共表达对IG1或IG2水平没有影响，说明所用制剂中的IL-6对任何测量的免疫反应没有调节作用。
