Omran Heymut, Loges Niki T
Department of Pediatrics and Adolescent Medicine, University Hospital Freiburg, 79106 Freiburg, Germany.
Methods Cell Biol. 2009;91:123-33. doi: 10.1016/S0091-679X(08)91007-4. Epub 2009 Dec 1.
Respiratory epithelial cells carry multiple motile cilia. Defective ciliary motility results in reduced mucociliary airway clearance in a destructive chronic airway disease referred to as primary ciliary dyskinesia. Immunofluorescence (IF) microscopy of respiratory cells allows visualization of proteins along the length of the ciliary axoneme and distinct compartments such as the transition zone and the basal body region at the ciliary base. The advantages of the technique to localize proteins such as axonemal dynein motor components along the ciliary axoneme and their diagnostic potential are shown. Special emphasis is placed on methodological subtleties and quality controls to assure accurate IF analyses.
呼吸道上皮细胞带有多个能动纤毛。纤毛运动功能缺陷会导致一种称为原发性纤毛运动障碍的破坏性慢性气道疾病中黏液纤毛气道清除功能降低。呼吸道细胞的免疫荧光（IF）显微镜检查可使沿纤毛轴丝长度以及纤毛基部诸如过渡区和基体区域等不同区室的蛋白质可视化。展示了该技术在沿纤毛轴丝定位诸如轴丝动力蛋白运动成分等蛋白质方面的优势及其诊断潜力。特别强调了方法上的细微之处和质量控制，以确保进行准确的IF分析。
