CNRS/Université de Pau, Laboratoire de Chimie Analytique Bio-Inorganique et Environnement, UMR 5254, Hélioparc, 2 Av du Président Angot, F-64053 Pau, France.
J Trace Elem Med Biol. 2010 Apr;24(2):124-9. doi: 10.1016/j.jtemb.2009.11.005. Epub 2010 Jan 21.
An Ussing chamber study was conducted in order to investigate the transport behavior of copper glycinate complexes through a porcine gastrointestinal membrane. Organic copper complexes such as copper tri- and tetraglycinates (GGG-Cu(II) and GGGG-Cu(II)) were used as model system. In a novel analytical approach the Ussing chamber was combined with mass spectrometry. Therefore, relevant analytical methods based on MALDI-MS and a coupling of capillary electrophoresis to ICP-MS and ESI-MS were developed for the determination of copper complexes in the mucosal and serosal half-chambers. It was found that 86.1+/-8.5% of copper triglycinate but only 20.8+/-9.9% of copper tetraglycinate penetrated the digestive membrane without modification. Furthermore, inorganic copper species were not detected but a new copper complex (m/z 442) was found to be formed in both compartments of the Ussing chamber.
进行了一项 Ussing 室研究，以调查甘氨酸铜配合物通过猪胃肠膜的转运行为。使用三甘氨酸铜（GGG-Cu(II)）和四甘氨酸铜（GGGG-Cu(II)）等有机铜配合物作为模型系统。在一种新的分析方法中，Ussing 室与质谱相结合。因此，基于 MALDI-MS 开发了相关的分析方法，并将毛细管电泳与 ICP-MS 和 ESI-MS 耦合，用于测定黏膜和浆膜半室中的铜配合物。结果发现，未经修饰时，86.1+/-8.5%的三甘氨酸铜穿透消化膜，但只有 20.8+/-9.9%的四甘氨酸铜穿透。此外，未检测到无机铜物种，但在 Ussing 室的两个隔室中均发现形成了一种新的铜配合物（m/z 442）。
