Genomics Research Center, Academia Sinica, 128 Sec. 2, Academic Road, Taipei, Taiwan.
Biomacromolecules. 2010 May 10;11(5):1308-13. doi: 10.1021/bm1000907.
We report on the synthesis and characterization of end-tethering polypeptide monolayers based on poly(beta-benzyl-L-aspartate) (PBLA) homopolymer and PBLA-b-poly(gamma-benzyl-L-glutamate) block copolymer. The homopolypeptide and copolypeptide brushes were fabricated by the sequential, surface-initiated vapor deposition polymerization of the N-carboxyanhydride of beta-benzyl-L-aspartate or gamma-benzyl-L-glutamate, yielding 80-nm-thick, chemically grafted films after 30 min of reaction time. Both Fourier transform infrared spectrometry and circular dichroism showed that the polypeptide brushes could be reversibly and repeatedly switched between left-handed and right-handed alpha-helical structures in response to solvent vapor exposure or permanently converted to a beta-sheet structure when heated to 160 degrees C in air. The facile, in vacuo manufacturability and the robustness of the films of PBLA-based brushes could allow them to be incorporated as active components for biosensing and nanofabricated devices.
我们报告了基于聚（β-苄基-L-天冬氨酸）（PBLA）均聚物和 PBLA-b-聚（γ-苄基-L-谷氨酸）嵌段共聚物的端接多肽单层的合成和表征。通过 N-羧酸酐的顺序、表面引发气相沉积聚合β-苄基-L-天冬氨酸或γ-苄基-L-谷氨酸，得到 80nm 厚的化学接枝膜，反应 30 分钟后。傅里叶变换红外光谱和圆二色性表明，多肽刷可以在溶剂蒸气暴露下可逆且反复地从左手和右手α-螺旋结构之间切换，或者在空气中加热至 160°C 时永久转换为β-折叠结构。基于 PBLA 的刷的易于在真空中制造和薄膜的坚固性可以使它们被整合为用于生物传感和纳米制造器件的活性成分。
