Department of Surgery, University of Utah School of Medicine, Salt Lake City, UT 84132, USA.
Pancreas. 2010 May;39(4):425-35. doi: 10.1097/MPA.0b013e3181c15963.
The dismal prognosis of pancreatic adenocarcinoma is due in part to a lack of molecular information regarding disease development. Established cell lines remain a useful tool for investigating these molecular events. Here we present a review of available information on commonly used pancreatic adenocarcinoma cell lines as a resource to help investigators select the cell lines most appropriate for their particular research needs. Information on clinical history; in vitro and in vivo growth characteristics; phenotypic characteristics, such as adhesion, invasion, migration, and tumorigenesis; and genotypic status of commonly altered genes (KRAS, p53, p16, and SMAD4) was evaluated. Identification of both consensus and discrepant information in the literature suggests careful evaluation before selection of cell lines and attention be given to cell line authentication.
胰腺腺癌预后不佳的部分原因是缺乏有关疾病发展的分子信息。已建立的细胞系仍然是研究这些分子事件的有用工具。在这里，我们回顾了常用胰腺腺癌细胞系的现有信息，以帮助研究人员选择最适合其特定研究需求的细胞系。评估了临床病史；体外和体内生长特性；表型特征，如粘附、侵袭、迁移和致瘤性；以及常见改变基因（KRAS、p53、p16 和 SMAD4）的基因型状态。文献中一致和不一致信息的识别表明，在选择细胞系之前需要仔细评估，并应注意细胞系的鉴定。
