Antonsen S, Pedersen F B, Wang P
Department of Clinical Chemistry, Odense University Hospital, Denmark.
Scand J Clin Lab Invest. 1991 Apr;51(2):149-54. doi: 10.1080/00365519109091101.
Biochemical detection of neutrophils in peritoneal effluents by Cytur-test has been advocated as a fast and reliable bedside test when peritonitis is suspected in patients on continuous ambulatory peritoneal dialysis. The Cytur-test developed a light blue colour when the neutrophil count exceeded 10(8) l-1 in the dialysis effluents, while in urine the same reaction is seen at a neutrophil concentration of 10(7) l-1. The Cytur-test was inhibited by glucose at 25 g l-1 and by albumin at 10 g l-1. However, the median (range) concentrations of glucose and albumin of 60 dialysis effluents was 1.8 g l-1 (0.9-10.6 g l-1) and 1.1 g l-1 (0.3-5.2 g l-1) respectively. Thus, presence of glucose and albumin cannot be the only reason of the reduced reactivity of the Cytur-test in dialysis effluents when compared with urine.
当怀疑持续非卧床腹膜透析患者发生腹膜炎时,Cytur检测法对腹膜透析液中的中性粒细胞进行生化检测,被认为是一种快速可靠的床旁检测方法。当透析液中中性粒细胞计数超过10⁸/L时,Cytur检测法会呈现浅蓝色,而在尿液中,当中性粒细胞浓度为10⁷/L时会出现相同反应。Cytur检测法会受到25g/L葡萄糖和10g/L白蛋白的抑制。然而,60份透析液中葡萄糖和白蛋白的中位数(范围)浓度分别为1.8g/L(0.9 - 10.6g/L)和1.1g/L(0.3 - 5.2g/L)。因此,与尿液相比,葡萄糖和白蛋白的存在并非Cytur检测法在透析液中反应性降低的唯一原因。