Ru(bpy)(3)(2+)-(CH(2))(n)-MV(2+) 配合物的合成及其 DNA 光解研究。
Synthesis and DNA photocleavage study of Ru(bpy)(3)(2+)-(CH(2))(n)-MV(2+) complexes.
机构信息
State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 158-40, Zhongshanlu, Xigang District, 116012, Dalian, China.
出版信息
Dalton Trans. 2010 May 14;39(18):4411-6. doi: 10.1039/b927568a. Epub 2010 Mar 29.
A series of Ru(bpy)(3)(2+)-(CH(2))(n)-MV(2+) complexes (, n = 3, 4, 7) used for DNA photocleavage have been designed and synthesized. Under the irradiation of visible light, complexes can cleave supercoiled plasmid DNA (pBR322) both in air and under Ar atmosphere. Radical species such as O(2)(-) , OH and the light-induced charge-separated (CS) oxidation state Ru(bpy)(3)(3+)-(CH(2))(n)-MV(+) are responsible for the cleavage. The longer the carbon chain linkage, the higher the DNA photocleavage efficiency. It is noted that backwards intramolecular electron transfer (ET) that exist in complexes can lead to some decreasing effect on the cleavage result, while inclusion of complexes with cucurbit[8]uril (CB[8]) inhibits the backwards ET to some extent, thereby increasing photocleavage efficiency.
已设计并合成了一系列用于 DNA 光解的 Ru(bpy)(3)(2+)-(CH(2))(n)-MV(2+) 配合物（n = 3、4、7）。在可见光照射下，配合物既能在空气中又能在 Ar 气氛下切割超螺旋质粒 DNA（pBR322）。O(2)(-)、OH 和光诱导的电荷分离（CS）氧化态 Ru(bpy)(3)(3+)-(CH(2))(n)-MV(+)等自由基物种负责切割。碳链连接越长，DNA 光解效率越高。值得注意的是，配合物中存在的反向分子内电子转移（ET）会对切割结果产生一些降低的影响，而加入葫芦[8]脲（CB[8]）的配合物在一定程度上抑制了反向 ET，从而提高了光解效率。
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