盘基网柄菌细胞膜对皂苷通透作用的抗性。
Resistance of Dictyostelium discoideum membranes to saponin permeabilization.
作者信息
Mercanti Valentina, Cosson Pierre
机构信息
Dpt of Cell Physiology and Metabolism, Geneva Faculty of Medicine, Centre Médical Universitaire, 1 rue Michel Servet, CH1211 Geneva 4, Switzerland.
出版信息
BMC Res Notes. 2010 Apr 28;3:120. doi: 10.1186/1756-0500-3-120.
BACKGROUND
Saponin is a mild detergent commonly used to permeabilize cells prior to immunofluorescence labeling of intracellular proteins. It has previously been used to that effect in Dictyostelium discoideum amoebae.
FINDINGS
We show that saponin, contrary to Triton X-100 or alcohol, permeabilizes at best incompletely membranes of Dictyostelium. In cells exposed to osmotic stress, almost complete resistance to saponin permeabilization was observed.
CONCLUSIONS
Saponin should be used with special care to permeabilize Dictyostelium membranes. This unsusual property is presumably linked to the specific sterol composition of Dictyostelium membranes. It may also represent an adaptation of Dictyostelium to harsh conditions or to natural compounds encountered in its natural environment.
背景
皂苷是一种温和的去污剂，常用于在对细胞内蛋白质进行免疫荧光标记之前使细胞通透。它此前已被用于对盘基网柄菌变形虫产生这种效果。
研究结果
我们发现，与 Triton X - 100 或酒精不同，皂苷最多只能使盘基网柄菌的细胞膜不完全通透。在受到渗透胁迫的细胞中，观察到对皂苷通透具有几乎完全的抗性。
结论
在使盘基网柄菌细胞膜通透时，应特别小心地使用皂苷。这种不寻常的特性可能与盘基网柄菌细胞膜的特定固醇组成有关。它也可能代表盘基网柄菌对恶劣条件或其天然环境中遇到的天然化合物的一种适应。
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