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盘基网柄菌细胞表面抗原的免疫荧光标记

Immunofluorescence labeling of cell surface antigens in Dictyostelium.

作者信息

Vernay Alexandre, Cosson Pierre

机构信息

Department of Cell Physiology and Metabolism, Geneva Faculty of Medicine, Centre Médical Universitaire, 1 rue Michel Servet, Geneva 4 CH1211, Switzerland.

出版信息

BMC Res Notes. 2013 Aug 12;6:317. doi: 10.1186/1756-0500-6-317.

DOI:10.1186/1756-0500-6-317
PMID:23937900
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3751103/
Abstract

BACKGROUND

Immunolocalization of cellular antigens typically requires fixation and permeabilization of cells, prior to incubation with antibodies.

FINDINGS

Assessing a test protein abundantly present at the cell surface of Dictyostelium cells, we show that in fixed cells, permeabilization extracts almost completely this cell surface antigen. The extent of this artifact is variable depending on the procedure used for labeling and permeabilization, as well as on the antigen considered.

CONCLUSIONS

An optimized protocol for labeling both surface and intracellular antigens without significant loss of labeling is proposed.

摘要

背景

细胞抗原的免疫定位通常需要在与抗体孵育之前对细胞进行固定和通透处理。

研究结果

在评估一种在盘基网柄菌细胞表面大量存在的测试蛋白时，我们发现，在固定细胞中，通透处理几乎完全提取了这种细胞表面抗原。这种假象的程度因用于标记和通透处理的程序以及所考虑的抗原而异。

结论

提出了一种优化方案，用于标记表面和细胞内抗原而不会有明显的标记损失。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b574/3751103/fd0e8a7b328e/1756-0500-6-317-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b574/3751103/48316baf7f0a/1756-0500-6-317-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b574/3751103/94b2831782ee/1756-0500-6-317-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b574/3751103/fd0e8a7b328e/1756-0500-6-317-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b574/3751103/48316baf7f0a/1756-0500-6-317-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b574/3751103/94b2831782ee/1756-0500-6-317-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b574/3751103/fd0e8a7b328e/1756-0500-6-317-3.jpg

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Glycosylation Modulates Plasma Membrane Trafficking of CD24 in Breast Cancer Cells.糖基化调节乳腺癌细胞中 CD24 的质膜运输。
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