Departamento de Genética, Universidade Federal de Pernambuco, Recife, PE, Brazil.
Rev Saude Publica. 2010 Jun;44(3):571-4. doi: 10.1590/s0034-89102010005000004. Epub 2010 Apr 30.
Diagnostic tests for American tegumentary leishmaniasis were performed on blood samples of patients living in two endemic municipalities in the state of Pernambuco, Northeastern Brazil. DNA was extracted from 119 samples and used as template for polymerase chain reaction (PCR) analysis. The tests used primers specific for the kinetoplast mini-circle DNA (kDNA) of Leishmania braziliensis, a species circulating in Pernambuco, which amplify a 750 base pair target sequence. In total, 58 subjects (48.7%) showed positive PCR amplification and 61 (51.3%) were negative. Of the PCR-positive samples, 37 ( congruent with 64%) were from treated, lesion-free subjects. In conclusion, the PCR technique is efficacious at identifying Leishmania DNA in biopsy and venous blood samples.
对生活在巴西东北部伯南布哥州两个流行地区的患者的血液样本进行了美国皮肤利什曼病的诊断检测。从 119 个样本中提取 DNA，并将其用作聚合酶链反应（PCR）分析的模板。该检测使用针对利什曼原虫巴西利什曼原虫（kinetoplast mini-circle DNA，kDNA）的引物，该种在伯南布哥州流行，可扩增 750 个碱基对的目标序列。总共有 58 名受试者（48.7％）的 PCR 呈阳性扩增，61 名（51.3％）为阴性。在 PCR 阳性样本中，有 37 个（占 64％）来自经过治疗且无病变的受试者。总之，PCR 技术可有效识别活检和静脉血样本中的利什曼原虫 DNA。
