Department of Pediatric Hematology and Oncology Research, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo, 157-8535, Japan.
Int J Hematol. 2010 Jun;91(5):808-19. doi: 10.1007/s12185-010-0567-z. Epub 2010 Apr 29.
B cell-activating factor belonging to the tumor necrosis factor superfamily (BAFF) is a crucial factor for B cell development and is involved in the survival of malignant B cells, but its effect on B cell precursors (BCPs) remains unclear. We investigated BCP acute lymphoblastic leukemia (-ALL) cells for BAFF receptor (-R) expression and compared the effect of BAFF on BCP-ALL cells and Burkitt lymphoma (BL) cells. Expression of BAFF-R was detected in some cell lines and some clinical specimens of both BL and BCP-ALL. BAFF acted on both BL and BCP-ALL cells and promoted proliferation by both. BAFF also inhibited apoptosis by BL cells induced by cross-linking of cell surface molecules and anticancer drugs, but failed to inhibit apoptosis by BCP-ALL cells. BAFF induced prompt and obvious activation of the NF-kappaB signaling pathway in BL cells, but only weak and delayed activation of the pathway in BCP-ALL cells. The results of this study indicate that some BCP-ALL cells and some BL cells express BAFF-R, but that the effects of BAFF on BCP-ALL cells are different from its effects on mature B cell malignancies.
B 细胞激活因子属于肿瘤坏死因子超家族（BAFF），是 B 细胞发育的关键因素，参与恶性 B 细胞的存活，但它对 B 细胞前体（BCP）的影响尚不清楚。我们研究了 BCP 急性淋巴细胞白血病（-ALL）细胞中 BAFF 受体（-R）的表达，并比较了 BAFF 对 BCP-ALL 细胞和 Burkitt 淋巴瘤（BL）细胞的影响。BAFF-R 的表达在一些细胞系和 BL 和 BCP-ALL 的一些临床标本中均有检测到。BAFF 作用于 BL 和 BCP-ALL 细胞，并通过两种方式促进增殖。BAFF 还可以抑制 BL 细胞表面分子和抗癌药物交联诱导的细胞凋亡，但不能抑制 BCP-ALL 细胞的凋亡。BAFF 诱导 BL 细胞中 NF-kappaB 信号通路的迅速而明显激活，但仅在 BCP-ALL 细胞中诱导较弱和延迟的通路激活。本研究结果表明，一些 BCP-ALL 细胞和一些 BL 细胞表达 BAFF-R，但 BAFF 对 BCP-ALL 细胞的作用与其对成熟 B 细胞恶性肿瘤的作用不同。
