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拟穴青蟹（甲壳纲：十足目：短尾亚目）脑和胸神经节中孕酮受体的鉴定与分布

The identification and distribution of progesterone receptors in the brain and thoracic ganglion in the mud crab Scylla paramamosain (Crustacea: Decapoda: Brachyura).

作者信息

Ye Haihui, Huang Huiyang, Song Ping, Wang Guizhong

机构信息

Department of Oceanography, State Key Laboratory of Marine Environmental Science, Xiamen University, 361005, Xiamen, China.

出版信息

Invert Neurosci. 2010 Nov;10(1):11-6. doi: 10.1007/s10158-010-0100-3. Epub 2010 Apr 29.

DOI:10.1007/s10158-010-0100-3
PMID:20429024
Abstract

The existence of progesterone receptors (PR) in the Scylla paramamosain (mud crab) was studied using immunological techniques. By Western blotting, PR with an apparent molecular weight of 70 kDa is identified in both the brain and the thoracic ganglion. By immunohistochemistry, PR immunoreactive neurons are detected mainly in the protocerebrum, the subesophageal ganglion and the leg ganglion. PR immunoreactivity is localized mainly in the nuclei of these neurons, while only a few neurons show such activities in their cytoplasm. Our results provide evidence that progesterone modulates the neuroendocrine system mainly via nucleus receptors.

摘要

运用免疫学技术研究了拟穴青蟹（泥蟹）中孕酮受体（PR）的存在情况。通过蛋白质免疫印迹法，在脑和胸神经节中均鉴定出了表观分子量为70 kDa的PR。通过免疫组织化学方法，PR免疫反应性神经元主要在原脑、咽下神经节和腿神经节中被检测到。PR免疫反应性主要定位于这些神经元的细胞核中，而只有少数神经元在其细胞质中显示出这种活性。我们的结果提供了证据表明孕酮主要通过核受体调节神经内分泌系统。

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Effects of exogenous progesterone and cloprostenol on ovarian follicular development and first ovulation in prepubertal heifers.外源性孕酮和氯前列醇对未成年小母牛卵巢卵泡发育和首次排卵的影响。
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