针对大肠杆菌O157:H7的单克隆抗体的制备与特性鉴定
Production and characterisation of monoclonal antibodies specific for Escherichia coli O157:H7.
作者信息
Luciani M, Armillotta G, Magliulo M, Portanti O, Di Febo T, Di Giannatale E, Roda A, Lelli R
机构信息
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale, Campo Boario, 64100 Teramo, Italy.
出版信息
Vet Ital. 2006 Jul-Sep;42(3):183-91, 173-82.
Seven monoclonal antibodies (MAbs) specific for Escherichia coli O157:H7, one of the major causes of haemorrhagic colitis in humans, were produced by immunising Balb/c mice with the strain E. coli O157:H7. These monoclonal antibodies do not cross-react with other bacteria such as Salmonella enterica serovar Typhimurium, E. coli O14, E. coli JM109, S. enterica serovar Enteritidis, S. panama, S. saintpaul, S. derby, S. muenchen, S. bredeney, S. hadar, Yersinia enterocolitica, Proteus vulgaris, Shigella flexneri, Listeria ivanovii, L. monocytogenes 13M, L. innocua, Enterobacter cloacae, E. agglomerans, E. amnigenus, Citrobacter freundii, Escherichia fergussoni or Klebsiella pneumoniae. Of the seven MAbs obtained, MAb 8B8C3 was selected to prepare a high-sensitivity sandwich ELISA method specific for O157:H7.
用大肠杆菌O157:H7菌株免疫Balb/c小鼠，产生了7种对人出血性结肠炎主要病因之一的大肠杆菌O157:H7具有特异性的单克隆抗体（MAb）。这些单克隆抗体与其他细菌无交叉反应，如肠炎沙门氏菌鼠伤寒血清型、大肠杆菌O14、大肠杆菌JM109、肠炎沙门氏菌肠炎血清型、巴拿马沙门氏菌、圣保罗沙门氏菌、德比沙门氏菌、慕尼黑沙门氏菌、布雷德尼沙门氏菌、哈达尔沙门氏菌、小肠结肠炎耶尔森氏菌、普通变形杆菌、福氏志贺氏菌、伊氏李斯特菌、单核细胞增生李斯特菌13M、无害李斯特菌、阴沟肠杆菌、聚团肠杆菌、羊水肠杆菌、弗氏柠檬酸杆菌、弗格森埃希氏菌或肺炎克雷伯菌。在获得的7种单克隆抗体中，选择单克隆抗体8B8C3来制备一种对O157:H7具有特异性的高灵敏度夹心酶联免疫吸附测定（ELISA）方法。
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