School of Public Health, Fudan University, Shanghai, China.
Epidemiol Infect. 2011 Jan;139(1):130-8. doi: 10.1017/S0950268810000890. Epub 2010 Apr 30.
This study investigated further the association between the Beijing family Mycobacterium tuberculosis circulating in rural China and anti-tuberculosis (TB) drug resistance. In total, 351 M. tuberculosis isolates were collected through a population-based epidemiological study, 223 (63·5%) of which were resistant to at least one anti-TB drug, including 53 (15·1%) multidrug-resistant (MDR) isolates. Spoligotyping found 243 isolates (69·2%) that belonged to the Beijing family. A major subgroup of the Beijing family identified by mycobacterial interspersed repetitive unit (MIRU) genotyping (223325173533), showed significantly higher frequencies of MDR (44·7% vs. 13·7%, OR 6·18, 95% CI 2·68-14·23), katG and rpoB mutations (31·6% vs. 9·3%, OR 4·27, 95% CI 1·86-9·80), and being clustered by IS6110 RFLP genotyping (60·5% vs. 21·0%, OR 6·14, 95% CI 2·82-13·37) in comparison with other Beijing family isolates. Our data suggest that MIRU genotype 223325173533 of the Beijing family is associated with MDR and increased transmissibility.
本研究进一步探讨了中国农村流行的北京家族分枝杆菌与抗结核（TB）药物耐药性之间的关联。通过一项基于人群的流行病学研究共收集了 351 株分枝杆菌分离株，其中 223 株（63.5%）至少对一种抗结核药物耐药，包括 53 株（15.1%）耐多药（MDR）分离株。 spoligotyping 发现 243 株（69.2%）属于北京家族。通过分枝杆菌插入重复单元（MIRU）基因分型确定的北京家族的一个主要亚群（223325173533），显示出更高的 MDR （44.7%对 13.7%，OR6.18，95%CI2.68-14.23）、katG 和 rpoB 突变（31.6%对 9.3%，OR4.27，95%CI1.86-9.80）以及 IS6110 RFLP 基因分型聚类的频率（60.5%对 21.0%，OR6.14，95%CI2.82-13.37）明显更高。我们的数据表明，北京家族的 MIRU 基因型 223325173533 与 MDR 和增加的传染性有关。
