Yule D I, Williams J A
Department of Physiology, University of Michigan Ann Arbor 48109-0622.
Biochem Biophys Res Commun. 1991 May 31;177(1):159-65. doi: 10.1016/0006-291x(91)91962-c.
Microfluorimetry of fura-2 was used to monitor [Ca2+]i in single cells stimulated with the G-protein activating agent mastoparan. Mastoparan induced the generation of [Ca2+]i oscillations, which in contrast to oscillations induced by low concentrations of CCK were acutely dependent on the presence of extracellular Ca2+. Oscillations were inhibited by phorbol ester. Sodium fluoride, a known activator of G-proteins, gave similar results. Both mastoparan and CCK induced turnover of inositol phosphates, at concentrations higher than necessary to induce oscillations.
使用fura-2微荧光测定法监测用G蛋白激活剂马斯托帕兰刺激的单细胞中的[Ca2+]i。马斯托帕兰诱导[Ca2+]i振荡的产生，与低浓度胆囊收缩素诱导的振荡相反，该振荡强烈依赖于细胞外Ca2+的存在。振荡被佛波酯抑制。已知的G蛋白激活剂氟化钠给出了类似的结果。马斯托帕兰和胆囊收缩素在高于诱导振荡所需的浓度下均诱导肌醇磷酸的周转。
