Kappe R, Rimek D
Institut für medizinische Laboratoriumsdiagnostik und Mikrobiologie, Südharz-Krankenhaus Nordhausen gGmbH, Nordhausen, Deutschland.
Mycoses. 2010 May;53 Suppl 1:26-9. doi: 10.1111/j.1439-0507.2009.01839.x.
Diseases caused by Aspergillus spp. are difficult to diagnose and thus require supplementary serological assays. This is the result of a selective review of the relevant literature with special regard to recent guidelines. In addition to conventional diagnostic tools (radiology, microscopy, culture) the measurement of the following serological markers is recommended, depending on the clinical type of aspergillosis: Invasive and chronic necrotising aspergillosis: Aspergillus-galactomannan antigen. Test format: EIA using the rat MAb EB-A2. Cut-off 0.5 (index). Monitoring of high risk patients: Twice weekly. Aspergillus-IgG (test format EIA) as confirmatory assay after recovery of the leukocyte function under therapy. Aspergilloma: Aspergillus IgG. Test format: EIA. Allergical aspergillosis: Aspergillus IgE. Test format: RAST. Galactomannan antigen detection rates high in the diagnosis of invasive aspergillosis. The evaluation of Aspergillus nucleic acid amplification assays is pending.
曲霉属真菌引起的疾病难以诊断，因此需要辅助血清学检测。这是对相关文献进行选择性综述并特别参考近期指南的结果。除了传统诊断工具（放射学、显微镜检查、培养）外，根据曲霉病的临床类型，建议检测以下血清学标志物：侵袭性和慢性坏死性曲霉病：曲霉半乳甘露聚糖抗原。检测形式：使用大鼠单克隆抗体EB - A2的酶免疫测定法。临界值0.5（指数）。对高危患者的监测：每周两次。曲霉IgG（检测形式为酶免疫测定法）作为治疗后白细胞功能恢复后的确认检测。曲菌球：曲霉IgG。检测形式：酶免疫测定法。过敏性曲霉病：曲霉IgE。检测形式：放射变应原吸附试验。半乳甘露聚糖抗原在侵袭性曲霉病诊断中的检出率较高。曲霉核酸扩增检测的评估尚在进行中。
