Mokhova E N, Skulachev V P, Zhigacheva I V
Biochim Biophys Acta. 1978 Mar 13;501(3):415-23. doi: 10.1016/0005-2728(78)90109-3.
15 min cold exposure of rats adapted to cold results in switching on a pathway of the fast oxidation of extramitochondrial NADH in the isolated liver mitochondria. This pathway is sensitive to mersalyl and cyanide, resistant to amytal and antimycin A, and can be stimulated by dinitrophenol. A portion of the endogenous cytochrome c pool can easily be removed by washing mitochondria of the cold-exposed rats. A scheme is discussed, postulating desorption of the inner membrane-bound cytochrome c into intermembrane space of mitochondria, resulting in formation of a link between the non-phosphorylating NADH-cytochrome c reductase in the outer mitochondrial membrane and cytochrome c oxidase in the inner membrane. It is suggested that such an oxidative pathway is involved in the urgent heat production in liver in response to the cold treatment.
对适应寒冷的大鼠进行15分钟的冷暴露，会导致分离的肝线粒体中一条线粒体外NADH快速氧化途径被开启。该途径对汞撒利和氰化物敏感，对戊巴比妥和抗霉素A有抗性，并且可被二硝基苯酚刺激。冷暴露大鼠的线粒体经洗涤后，内源性细胞色素c池的一部分可轻易去除。本文讨论了一个假说，即内膜结合的细胞色素c解吸进入线粒体的膜间隙，导致线粒体外膜中无磷酸化作用的NADH-细胞色素c还原酶与内膜中的细胞色素c氧化酶之间形成联系。有人提出，这种氧化途径参与了肝脏对冷处理作出的紧急产热过程。
