Faculty of Agriculture, Shizuoka University, Surugaku, Shizuoka 422-8529, Japan.
J Hazard Mater. 2010 Aug 15;180(1-3):764-7. doi: 10.1016/j.jhazmat.2010.04.024. Epub 2010 Apr 13.
The antimicrobial and preservative agent triclosan (TCS) is an emerging and persistent pollutant with a ubiquitous presence in the aquatic environment. Thus, TCS was treated with manganese peroxidase (MnP), laccase and the laccase-mediator system with 1-hydroxybenzotriazole. MnP was most effective in eliminating TCS among the three enzymatic treatments, with TCS concentration being reduced by about 94% after 30 min following treatment with 0.5 nkat mL(-1) MnP and being almost completely eliminated after 60 min. Furthermore, MnP (0.5 nkat mL(-1)) caused the complete loss of bacterial growth inhibition by TCS after 30 min and reduced the algal growth inhibition of TCS by 75% and 90% after 30 and 60 min, respectively. These results strongly suggest that MnP is effective in removing the ecotoxicity of TCS.
三氯生（TCS）作为一种新型的、普遍存在于水环境中的持久性抗菌防腐剂，属于新兴污染物。本研究采用锰过氧化物酶（MnP）、漆酶及其与 1-羟基苯并三唑组成的酶促体系对 TCS 进行处理。在这三种酶处理方法中，MnP 对 TCS 的去除效果最为显著，在 0.5 nkat·mL-1 MnP 处理 30 min 后，TCS 浓度降低了约 94%，60 min 后几乎完全消除。此外，MnP（0.5 nkat·mL-1）在 30 min 内完全消除了 TCS 对细菌生长的抑制作用，并分别在 30 和 60 min 时将 TCS 对藻类生长的抑制率降低了 75%和 90%。这些结果强烈表明 MnP 能有效去除 TCS 的生态毒性。
