Department of Chemistry, Wayne State University, Detroit, Michigan 48202, USA.
J Am Soc Mass Spectrom. 2010 Jul;21(7):1260-4. doi: 10.1016/j.jasms.2010.03.039. Epub 2010 Apr 3.
A simple device is described for desolvation of highly charged matrix/analyte clusters produced by laser ablation leading to multiply charged ions that are analyzed by ion mobility spectrometry-mass spectrometry. Thus, for example, highly charged ions of ubiquitin and lysozyme are cleanly separated in the gas phase according to size and mass (shape and molecular weight) as well as charge using Tri-Wave ion mobility technology coupled to mass spectrometry. This contribution confirms the mechanistic argument that desolvation is necessary to produce multiply charged matrix-assisted laser desorption/ionization (MALDI) ions and points to how these ions can be routinely formed on any atmospheric pressure mass spectrometer.
本文描述了一种简单的装置，用于对激光烧蚀产生的高电荷基质/分析物簇进行去溶剂化处理，从而得到多电荷离子，然后通过离子淌度谱-质谱进行分析。例如，使用三波离子淌度技术与质谱联用，可以根据大小和质量（形状和分子量）以及电荷，将泛素和溶菌酶的高电荷离子在气相中进行干净的分离。本研究证实了去溶剂化对于产生多电荷基质辅助激光解吸/电离（MALDI）离子的必要性，并指出了如何在任何大气压质谱仪上常规形成这些离子。
