UMR M Ifremer, Physiologie et Ecophysiologie des Mollusques Marins, IFR ICORE, Université de Caen Basse-Normandie, France.
Comp Biochem Physiol Part D Genomics Proteomics. 2010 Jun;5(2):157-64. doi: 10.1016/j.cbd.2010.03.004. Epub 2010 Apr 2.
In the dogfish (Scyliorhinus canicula L.) the testicular germinative zone (GZ), composed of large isolated spermatogonia surrounded by elongating pre-Sertoli cells, is located between the albuginea and the ventrolateral intratesticular vessel. During the spermatogenic wave, cysts radiate in maturational order forming distinct testicular zones. In this study, soluble proteins of the GZ and of the zone containing cysts with spermatocytes were separated by two-dimensional electrophoresis. Gel images were matched and then evaluated for GZ-specific proteins. From the1400 protein spots identified, 680 were found to be apparently specific to this zone. Using MALDI-TOF/TOF mass spectrometry, de novo sequences were obtained for 33 proteins out of the 169 selected for identification by mass spectrometry, but only 16 of these 169 proteins were identified. One of them, proteasome subunit alpha-6, was analyzed further by immunohistochemistry. This study demonstrates the utility of the dogfish as a model for proteome analysis of the spermatogonial stem cell niche, even if it remains restricted by the lack of genomic data available on Elasmobranchs.
在星鲨（Scyliorhinus canicula L.）中，生精上皮区（GZ）由大而孤立的精原细胞组成，周围环绕着伸长的支持细胞，位于白膜和腹外侧睾丸内血管之间。在精子发生波期间，小囊以成熟顺序辐射状排列，形成明显的睾丸区。在这项研究中，通过二维电泳分离了 GZ 和含有精母细胞小囊的区的可溶性蛋白质。对凝胶图像进行匹配和评估，以确定 GZ 特异性蛋白质。在所鉴定的 1400 个蛋白质斑点中，有 680 个明显特异性地存在于该区域。使用 MALDI-TOF/TOF 质谱法，从 169 个经质谱法鉴定选择的蛋白质中获得了 33 个蛋白质的从头序列，但仅鉴定出其中的 16 个蛋白质。其中之一，蛋白酶体亚基α-6，通过免疫组织化学进一步分析。这项研究证明了星鲨作为精原干细胞龛蛋白质组分析模型的实用性，即使它仍然受到缺乏软骨鱼类基因组数据的限制。
