Fabiato A, Fabiato F
Biochim Biophys Acta. 1978 Mar 1;539(2):253-60. doi: 10.1016/0304-4165(78)90012-0.
In the presence of low concentrations of total EGTA (5 . 10(-4) M) and free Mg2+ (3.16 . 10(-5) M) and in the presence of caffeine (8 . 10(-3) M), cyclic AMP (5 . 10(-6) M) produces a relaxation of the tension developed by skinned fibres from cat caudo-femoralis. The relaxation can be attributed to an enhancement of the Ca2+ accumulation by the sarcoplasmic reticulum, since cyclic AMP does not modify the sensitivity of the myofilaments of Ca2+. These results are similar to those previously reported for the effect of cyclic AMP on skinned cardiac cells in the presence of a higher free Mg2+ concentration and in the absence of caffeine. This similarity suggests that the mode of action of cyclic AMP on the sarcoplasmic reticulum is not fundamentally different in cardiac and fast skeletal muscles.
在存在低浓度的总乙二醇双四乙酸（5×10⁻⁴ M）和游离镁离子（3.16×10⁻⁵ M）以及咖啡因（8×10⁻³ M）的情况下，环磷酸腺苷（5×10⁻⁶ M）会使猫尾股肌的去皮纤维产生的张力松弛。这种松弛可归因于肌浆网对钙离子摄取的增强，因为环磷酸腺苷不会改变肌丝对钙离子的敏感性。这些结果与先前报道的在较高游离镁离子浓度且无咖啡因存在的情况下环磷酸腺苷对去皮心肌细胞的作用结果相似。这种相似性表明，环磷酸腺苷对肌浆网的作用方式在心肌和快速骨骼肌中并无根本差异。
