Ley K, Gaehtgens P, Fennie C, Singer M S, Lasky L A, Rosen S D
Department of Physiology, Freie Universität Berlin, Germany.
Blood. 1991 Jun 15;77(12):2553-5.
During the inflammatory response, granulocytes and other leukocytes adhere to and emigrate from small venules. Before firm attachment, leukocytes are observed rolling slowly along the endothelium in venules of most tissues accessible to intravital microscopy. The molecular mechanism underlying this early type of leukocyte-endothelial interaction is unknown. Leukocyte rolling was investigated in venules (diameter, 40 microns) of the exposed rat mesentery. Micro-infusion of a recombinant soluble chimera (LEC-IgG) of the murine homing receptor lectin-like cell adhesion molecule 1 (LEC-CAM 1; gp90MEL) into individual venules reduced the number of rolling leukocytes by 89% +/- 2% (mean +/- SEM, n = 20 venules), while a similar CD4 chimera (CD4-IgG) had no effect (inhibition 14% +/- 7%, n = 25). Rolling was also greatly reduced by a polyclonal serum against LEC-CAM 1 (inhibition 84% +/- 3%, n = 35); preimmune serum was ineffective (11% +/- 13% inhibition, n = 28). These findings indicate that LEC-CAM 1 mediates the adhesive interaction underlying leukocyte rolling and thus may play an important role in inflammation and in pathologic conditions involving leukocytes.
在炎症反应过程中，粒细胞和其他白细胞会黏附于小静脉并从小静脉游出。在牢固黏附之前，在大多数活体显微镜可观察的组织的小静脉中，可观察到白细胞沿内皮缓慢滚动。这种早期白细胞与内皮细胞相互作用的分子机制尚不清楚。在暴露的大鼠肠系膜的小静脉（直径40微米）中研究了白细胞滚动。将鼠归巢受体凝集素样细胞黏附分子1（LEC-CAM 1；gp90MEL）的重组可溶性嵌合体（LEC-IgG）微量注入单个小静脉，可使滚动白细胞数量减少89%±2%（平均值±标准误，n = 20条小静脉），而类似的CD4嵌合体（CD4-IgG）则无作用（抑制率14%±7%，n = 25）。抗LEC-CAM 1的多克隆血清也可使滚动显著减少（抑制率84%±3%，n = 35）；免疫前血清无效（抑制率11%±13%，n = 28）。这些发现表明，LEC-CAM 1介导白细胞滚动的黏附相互作用，因此可能在炎症和涉及白细胞的病理状况中起重要作用。
