Hayward C P, Warkentin T E, Horsewood P, Kelton J G
Department of Medicine, McMaster University Medical Centre, Hamilton, Ontario, Canada.
Blood. 1991 Jun 15;77(12):2556-60.
Platelets contain proteins with biochemical properties that are well adapted to promoting hemostasis. One important adhesive protein is von Willebrand factor (vWF), which is a very large protein comprised of a series of multimers, ranging from 860,000 to over 10 million daltons. In this report we describe a second platelet protein, p-155, which has a similar unique multimeric composition. Using agarose-acrylamide gel electrophoresis, platelet p-155 was shown to be composed of multimers ranging from less than 450 Kd to many million daltons. Based on this unique structure, we propose that the native molecule be designated as multimerin. Comparison with vWF showed that multimerin contained less of the very high molecular weight multimers. Differential reduction demonstrated that the smallest multimer is a trimer, composed of three 155-Kd subunits. Platelet releasate was demonstrated to contain mainly the smaller multimers, suggesting that the larger multimers bind to the platelet surface. Other studies indicate that multimerin and vWF are the two largest platelet proteins and the only two platelet proteins exhibiting a complex, disulfide-linked multimeric composition with variability in multimer size.
血小板含有具有生化特性的蛋白质，这些特性非常适合促进止血。一种重要的粘附蛋白是血管性血友病因子（vWF），它是一种非常大的蛋白质，由一系列多聚体组成，分子量从860,000到超过1000万道尔顿不等。在本报告中，我们描述了第二种血小板蛋白p - 155，它具有类似的独特多聚体组成。使用琼脂糖 - 丙烯酰胺凝胶电泳，血小板p - 155被证明由分子量小于450 Kd到数百万道尔顿的多聚体组成。基于这种独特结构，我们建议将天然分子命名为多聚素。与vWF的比较表明，多聚素含有的高分子量多聚体较少。差异还原表明，最小的多聚体是三聚体，由三个155 - Kd亚基组成。血小板释放物被证明主要含有较小的多聚体，这表明较大的多聚体与血小板表面结合。其他研究表明，多聚素和vWF是两种最大的血小板蛋白，也是仅有的两种呈现复杂的、二硫键连接的多聚体组成且多聚体大小可变的血小板蛋白。
