采用液相色谱-串联质谱法测定大鼠血浆中对氨基马尿酸的含量。
Determination of p-aminohippuric acid in rat plasma by liquid chromatography-tandem mass spectrometry.
机构信息
School of Pharmacy, National Defense Medical Center, Taipei, Taiwan, ROC.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jun 1;878(19):1643-6. doi: 10.1016/j.jchromb.2010.04.015. Epub 2010 Apr 24.
A rapid, simple and sensitive method was developed for the determination of para-aminohippuric acid (PAH) in rat plasma using liquid chromatography tandem mass spectrometry (LC-MS-MS). Acetaminophen was used as the internal standard. Chromatographic separation was performed using a Symmetry C18 column and the mobile phase was composed of A: 2 mM ammonium formate and 0.1% formic acid in water and B: 2 mM ammonium formate and 0.1% formic acid in acetonitrile (ACN) (A:B, 30:70, v/v). Detection was performed on a triple-quadrupole tandem mass spectrometer using positive ion mode electrospray ionization (ESI) in the multiple reaction monitoring (MRM) mode. The MS/MS ion transitions monitored were m/z 195.2-->120.2 and 152.1-->110.1 for PAH and acetaminophen, respectively. Good linearity is observed over the concentration range of 0.1-500 microg/ml. The method was proved to be accurate and reliable and was applied to a pharmacokinetic study in rat.
建立了一种灵敏、快速、简单的测定大鼠血浆中对氨基马尿酸（PAH）的 LC-MS-MS 方法。采用乙腈-水（30:70，V/V）作为流动相，以 2mM 甲酸铵和 0.1%甲酸水溶液（A）-2mM 甲酸铵和 0.1%甲酸乙腈溶液（B）作为梯度洗脱，以 152.1-->110.1（内标）和 195.2-->120.2（PAH）为监测离子，正离子模式电喷雾离子源（ESI），多反应监测（MRM）方式检测。PAH 在 0.1-500μg/ml 浓度范围内线性关系良好。方法准确、可靠，适用于大鼠的药代动力学研究。
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