果蝇 Chd3 基因和多线染色体 76B-D 区的分子遗传学分析。
Molecular genetic analysis of Chd3 and polytene chromosome region 76B-D in Drosophila melanogaster.
机构信息
Program in Genomics of Differentiation, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-2785, USA.
出版信息
Genetics. 2010 Jul;185(3):811-22. doi: 10.1534/genetics.110.115121. Epub 2010 May 3.
The Drosophila melanogaster Chd3 gene encodes a member of the CHD group of SNF2/RAD54 ATPases. CHD proteins are conserved from yeast to man and many are subunits of chromatin-remodeling complexes that facilitate transcription. Drosophila CHD3 proteins are not found in protein complexes, but as monomers that remodel chromatin in vitro. CHD3 colocalize with elongating RNA polymerase II on salivary gland polytene chromosomes. Since the role of Chd3 in development was unknown, we isolated and characterized the essential genes within the 640-kb region of the third chromosome (polytene chromosome region 76B-D) that includes Chd3. We recovered mutations in 24 genes that are essential for zygotic viability. We found that transposon-insertion mutants for 46% of the essential genes are included in the Drosophila Gene Disruption Project collection. None of the essential genes that we identified are in a 200-kb region that includes Chd3. We generated a deletion of Chd3 by targeted gene replacement. This deletion had no effect on either viability or fertility.
果蝇中的 Chd3 基因编码一个 SNF2/RAD54 ATPases 家族的 CHD 蛋白。CHD 蛋白在从酵母到人之间都具有保守性，其中许多都是染色质重塑复合物的亚基，这些复合物有助于转录。果蝇 CHD3 蛋白并不存在于蛋白质复合物中，而是作为单体在体外重塑染色质。CHD3 与正在延伸的 RNA 聚合酶 II 在唾液腺多线染色体上共定位。由于 Chd3 在发育中的作用尚不清楚，我们在包含 Chd3 的第三染色体（多线染色体区域 76B-D）的 640kb 区域内分离并鉴定了必需基因。我们在 24 个必需基因中发现了突变，这些突变对于合子的存活是必需的。我们发现，转座子插入突变的必需基因中有 46%包含在果蝇基因敲除计划的收集品中。我们鉴定的必需基因中没有一个位于包含 Chd3 的 200kb 区域内。我们通过靶向基因替换生成了 Chd3 的缺失。该缺失对活力或育性均没有影响。
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