Monsalve-Castillo Francisca, Chacín-Bonilla Leonor, Atencio Ricardo José, Porto Leticia Denys, Costa-León Luciana Ana, Estévez Jesús Enrique, Callejas-Valero Diana Estela
Cátedra de Virología, Escuela de Bioanálisis, Facultad de Medicina, Universidad del Zulia, Maracaibo, Estado Zulia, Venezuela.
Biomedica. 2009 Dec;29(4):647-52.
The high risk behaviors observed in prison centers have favored the transmission of hepatitis C virus infection. The main risk factor to acquire hepatitis C virus infection seems to be the use of intravenous drugs. In Venezuela, the prevalence of the infection in these centers is unknown since studies of the hepatitis C virus there are lacking.
The aim of this study was to determine the prevalence of hepatitis C virus and the risk factors involved in the transmission in prisoner populations.
A sample of 200 prisoners was studied from Sabaneta Jail, Maracaibo, Venezuela. The ages were between 18-69 years (average +/- DS: 31.6+/-9.9 years). Serum samples were tested by a fourth generation enzyme-linked immunosorbent assay ELISA and a confirmatory assay INNO-LIA. Both kits were from Innogenetic Laboratories N.V. (Belgium). Viral RNA was tested by the reverse transcription polymerase chain reaction technique (RT-PCR).
The ELISA assay determined a hepatitis C virus prevalence of 5.0% (10/200); 3/200 (1.5%) individuals were positive by both INNO-LIA and RT-PCR tests.
The observed prevalence of hepatitis C virus antibodies in this population was very low, suggesting a low circulation of the virus in this environment and a low level of associated risk behaviors.
在监狱中心观察到的高风险行为助长了丙型肝炎病毒感染的传播。感染丙型肝炎病毒的主要危险因素似乎是静脉注射毒品的使用。在委内瑞拉，由于缺乏对丙型肝炎病毒的研究，这些中心的感染患病率尚不清楚。
本研究的目的是确定丙型肝炎病毒的患病率以及囚犯群体中传播所涉及的危险因素。
对来自委内瑞拉马拉开波萨巴内塔监狱的200名囚犯进行了抽样研究。年龄在18 - 69岁之间（平均±标准差：31.6±9.9岁）。血清样本通过第四代酶联免疫吸附测定法（ELISA）和确证测定法INNO-LIA进行检测。两种试剂盒均来自比利时的Innogenetic Laboratories N.V.。通过逆转录聚合酶链反应技术（RT-PCR）检测病毒RNA。
ELISA测定法确定丙型肝炎病毒患病率为5.0%（10/200）；3/200（1.5%）的个体通过INNO-LIA和RT-PCR检测均为阳性。
在该人群中观察到的丙型肝炎病毒抗体患病率非常低，表明该病毒在这种环境中的传播率较低，且相关风险行为水平较低。
