School of Pharmacy, Fudan University, Shanghai, China.
Talanta. 2010 Jun 15;81(4-5):1325-30. doi: 10.1016/j.talanta.2010.02.028. Epub 2010 Feb 13.
A simple method based on disposable gelatin gel templates has been developed for the low-cost fabrication of poly(methyl methacrylate) (PMMA) microfluidic chips. Gelatin was dissolved in glycerol aqueous solution under heat to prepare a thermally reversible impression material. The molten gel was then sandwiched between a glass plate and a SU-8 template bearing negative relief of microstructure. After cooling, the negative SU-8 template could be easily separated from the solidified gelatin gel and a layer of gelatin template bearing positive relief of the microstructure was left on the glass plate. Subsequently, prepolymerized methyl methacrylate molding solution containing a UV-initiator was sandwiched between the gel template and a PMMA plate and was allowed to polymerize under UV light to fabricate PMMA channel plate at room temperature. Complete microchips could be obtained by bonding the channel plates with covers using plasticizer-assisted thermal bonding at 90 degrees C. Gelatin gel template can be mass-produced and will find application in the mass production of PMMA microchips at low cost. The prepared microfluidic microchips have been successfully employed in the capillary electrophoresis analysis of several ions in connection with contactless conductivity detection.
一种基于一次性明胶凝胶模板的简单方法已经被开发出来，用于低成本制造聚甲基丙烯酸甲酯（PMMA）微流控芯片。明胶在甘油水溶液中加热溶解，制备热可逆的印模材料。然后将熔融凝胶夹在玻璃板和带有负微结构浮雕的 SU-8 模板之间。冷却后，负的 SU-8 模板可以很容易地从凝固的明胶凝胶中分离出来，并且在玻璃板上留下一层带有正微结构浮雕的明胶模板。随后，将含有光引发剂的预聚物甲基丙烯酸甲酯成型溶液夹在凝胶模板和 PMMA 板之间，并在紫外光下聚合，在室温下制造 PMMA 通道板。通过在 90 摄氏度下使用增塑剂辅助热键合将通道板与盖子粘合，可以获得完整的微芯片。明胶凝胶模板可以批量生产，并将在低成本批量生产 PMMA 微芯片方面得到应用。所制备的微流控微芯片已成功用于与非接触式电导检测相结合的几种离子的毛细管电泳分析。
