Lin P S, Butterfield C E, Wallach F H
Cell Biol Int Rep. 1978 Jan;2(1):11-8. doi: 10.1016/0309-1651(78)90079-6.
Explants of simian virus 40 (SV40)-induced lymphoid tumors yield SV40-T-antigen-positive derivatives that differ from GD248 lymphocytes propagated in suspension culture, (or in vivo), in the following respects: polygonal shape, adhesion to culture substrates in vitro, phagocytic capacity, lack of immunoglobulin and a chromosome complement at least twice that of GD248 lymphocytes. When GD-248 lymphocytes are propagated as suspension in vitro, no such adherent variants can be detected. However, sequential in vivo passage of GD248 lymphocytes obtained from the suspension-culture lines also yield adherent cell lines upon explanation in vitro. Injection of adherent cells into hamsters produces tumors with histological features of reticulum cell sarcoma.
猿猴病毒40（SV40）诱导的淋巴肿瘤外植体产生SV40-T抗原阳性衍生物，这些衍生物在以下方面与悬浮培养（或体内）繁殖的GD248淋巴细胞不同：多边形形状、体外对培养底物的粘附性、吞噬能力、缺乏免疫球蛋白以及染色体数量至少是GD248淋巴细胞的两倍。当GD-248淋巴细胞在体外作为悬浮液繁殖时，无法检测到此类贴壁变体。然而，从悬浮培养系获得的GD248淋巴细胞经体内连续传代后，在体外培养时也会产生贴壁细胞系。将贴壁细胞注射到仓鼠体内会产生具有网状细胞肉瘤组织学特征的肿瘤。
