Department of Food Science and Human Nutrition, University of Florida/IFAS, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL, USA.
Biotechnol Lett. 2010 Sep;32(9):1287-91. doi: 10.1007/s10529-010-0288-7. Epub 2010 May 5.
Lipase-catalyzed synthesis of isoamyl acetate in hexane at 10-250 MPa at 80 degrees C and 1-100 MPa at 40 degrees C resulted in activation volumes of -12.9 +/- 1.7 and -21.6 +/- 2.9 cm(3) mol(-1), respectively. Increasing pressure from 10 to 200 MPa resulted in approximately 10-fold increase in V(max) at both 40 and 80 degrees C. Pressure increased the K(m) from 2.4 +/- 0.004 to 38 +/- 0.78 mM at 40 degrees C. In contrast, at 80 degrees C the pressure did not affect the K(m).
在 80°C 下，10-250 MPa 及在 40°C 下 1-100 MPa 的条件下，脂肪酶催化己烷中的乙酸异戊酯合成，分别得到活化体积-12.9±1.7 和-21.6±2.9 cm3/mol。在 40°C 和 80°C 下，压力从 10 MPa 增加到 200 MPa 导致 Vmax 约增加了 10 倍。压力将 K(m)从 40°C 的 2.4±0.004 增加到 38±0.78 mM。相比之下，在 80°C 下，压力不影响 K(m)。
