金黄色葡萄球菌6-羟甲基-7,8-二氢蝶呤焦磷酸激酶的结晶及初步X射线分析
Crystallization and preliminary X-ray analysis of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase from Staphylococcus aureus.
作者信息
Chhabra Sandeep, Newman Janet, Peat Thomas S, Fernley Ross T, Caine Joanne, Simpson Jamie S, Swarbrick James D
机构信息
Medicinal Chemistry and Drug Action, Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, Victoria 3052, Australia.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1;66(Pt 5):575-8. doi: 10.1107/S1744309110010857. Epub 2010 Apr 30.
6-Hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) catalyzes the Mg(2+)-dependent transfer of pyrophosphate from ATP to 6-hydroxymethyl-7,8-dihydropterin (HMDP), forming 6-hydroxymethyl-7,8-dihydropterin pyrophosphate, which is a critical step in the de novo folic acid-biosynthesis pathway. Diffraction-quality crystals of HPPK from the medically relevant species Staphylococcus aureus were grown in the presence of ammonium sulfate or sodium malonate and diffracted to better than 1.65 A resolution. The crystals belonged to space group P2(1), with unit-cell parameters a = 36.8, b = 76.6, c = 51.5 A, alpha = gamma = 90.0, beta = 100.2 degrees . The crystals contained two molecules per asymmetric unit, with a volume per protein weight (V(M)) of 2.04 A(3) Da(-1) and an estimated solvent content of 39.6%.
6-羟甲基-7,8-二氢蝶呤焦磷酸激酶（HPPK）催化焦磷酸从ATP到6-羟甲基-7,8-二氢蝶呤（HMDP）的镁离子依赖性转移，形成6-羟甲基-7,8-二氢蝶呤焦磷酸，这是从头合成叶酸生物合成途径中的关键步骤。在硫酸铵或丙二酸钠存在下，培养出了来自医学相关物种金黄色葡萄球菌的HPPK的高质量衍射晶体，其衍射分辨率优于1.65 Å。晶体属于空间群P2(1)，晶胞参数为a = 36.8、b = 76.6、c = 51.5 Å，α = γ = 90.0，β = 100.2°。每个不对称单元包含两个分子，每蛋白重量的体积（V(M)）为2.04 ų Da⁻¹，估计溶剂含量为39.6%。
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