Nuemket Nipawan, Tanaka Yoshikazu, Tsukamoto Kentaro, Tsuji Takao, Nakamura Keiji, Kozaki Shunji, Yao Min, Tanaka Isao
Graduate School of Life Sciences, Hokkaido University, Sapporo 060-0810, Japan.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1;66(Pt 5):608-10. doi: 10.1107/S1744309110012182. Epub 2010 Apr 30.
Botulinum toxin (BoNT) from Clostridium botulinum OFD05, isolated from bovine botulism, is a D/C mosaic-type BoNT. BoNTs possess binding, translocation and catalytic domains. The BoNT/OFD05 binding domain exhibits significant sequence identity to BoNT/C, which requires a single ganglioside as a binding receptor on neuronal cells, while BoNT/A and BoNT/B require two receptors for specific binding. To determine the binding mechanism of BoNT/OFD05 and its ganglioside receptors on neuronal cells, recombinant BoNT/OFD05 receptor-binding domain has been expressed, purified and crystallized. Native and SeMet-derivative crystals showed X-ray diffraction to 2.8 and 3.1 A resolution, respectively. The crystals belonged to space group P2(1)2(1)2(1).
从牛肉毒中毒中分离出的来自肉毒梭菌OFD05的肉毒杆菌毒素（BoNT）是一种D/C镶嵌型BoNT。BoNTs具有结合、转位和催化结构域。BoNT/OFD05结合结构域与BoNT/C具有显著的序列同一性，BoNT/C在神经元细胞上需要单一神经节苷脂作为结合受体，而BoNT/A和BoNT/B需要两种受体进行特异性结合。为了确定BoNT/OFD在神经元细胞上的结合机制及其神经节苷脂受体，已表达、纯化并结晶了重组BoNT/OFD05受体结合结构域。天然晶体和SeMet衍生晶体的X射线衍射分辨率分别为2.8 Å和3.1 Å。晶体属于空间群P2(1)2(1)2(1)。
