通过分离对线粒体丙酮酸转运抑制剂α-反式氰基肉桂酸乙酯具有抗性的突变体来培育低丙酮酸产生的清酒酵母。
Breeding of a low pyruvate-producing sake yeast by isolation of a mutant resistant to ethyl alpha-transcyanocinnamate, an inhibitor of mitochondrial pyruvate transport.
作者信息
Horie Kenta, Oba Takahiro, Motomura Saori, Isogai Atsuko, Yoshimura Takashi, Tsuge Keisuke, Koganemaru Kazuyoshi, Kobayashi Genta, Kitagaki Hiroshi
机构信息
Faculty of Agriculture, Saga University, Japan.
出版信息
Biosci Biotechnol Biochem. 2010;74(4):843-7. doi: 10.1271/bbb.90373.
Pyruvate is the key substance controlling the formation of diacetyl, acetaldehyde, and acetate during alcoholic fermentation. Here we report the breeding of a low pyruvate-producing sake yeast by isolation of a mutant resistant to ethyl alpha-transcyanocinnamate, an inhibitor of mitochondrial pyruvate transport. Mitochondrial function was involved in resistance to this substance and in the production of pyruvate by the mutants.
丙酮酸是酒精发酵过程中控制双乙酰、乙醛和乙酸形成的关键物质。在此，我们报告了一种低丙酮酸产量的清酒酵母的培育方法，该方法是通过分离对线粒体丙酮酸转运抑制剂α-反式氰基肉桂酸乙酯具有抗性的突变体来实现的。线粒体功能与对该物质的抗性以及突变体丙酮酸的产生有关。
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