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酿酒酵母中线粒体丙酮酸载体的鉴定。

Identification of the mitochondrial pyruvate carrier in Saccharomyces cerevisiae.

作者信息

Hildyard John C W, Halestrap Andrew P

机构信息

Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK.

出版信息

Biochem J. 2003 Sep 15;374(Pt 3):607-11. doi: 10.1042/BJ20030995.

DOI:10.1042/BJ20030995
PMID:12887330
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1223651/
Abstract

Mitochondrial pyruvate transport is fundamental for metabolism and mediated by a specific inhibitable carrier. We have identified the yeast mitochondrial pyruvate carrier by measuring inhibitor-sensitive pyruvate uptake into mitochondria from 18 different Saccharomyces cerevisiae mutants, each lacking an unattributed member of the mitochondrial carrier family (MCF). Only mitochondria from the YIL006w deletion mutant exhibited no inhibitor-sensitive pyruvate transport, but otherwise behaved normally. YIL006w encodes a 41.9 kDa MCF member with homologous proteins present in both the human and mouse genomes.

摘要

线粒体丙酮酸转运对于新陈代谢至关重要，且由一种特定的可抑制载体介导。我们通过测量18种不同的酿酒酵母突变体线粒体对抑制剂敏感的丙酮酸摄取量，鉴定出了酵母线粒体丙酮酸载体，每个突变体都缺失线粒体载体家族（MCF）中一个未明确归属的成员。只有YIL006w缺失突变体的线粒体未表现出对抑制剂敏感的丙酮酸转运，但在其他方面表现正常。YIL006w编码一个41.9 kDa的MCF成员，在人类和小鼠基因组中都存在同源蛋白。

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