State Key Laboratory of Agro-biotechnology and Department of Plant Pathology, China Agricultural University, Beijing, China.
Arch Virol. 2010 Jun;155(6):949-57. doi: 10.1007/s00705-010-0672-6. Epub 2010 May 6.
A potyvirus causing distortion and mosaic symptoms in the herbal plant Sanqi (Panax notoginseng) was isolated from Yunnan province, China, and the complete nucleotide sequence of one isolate and the partial sequences of two other isolates were determined. The viral RNA genome comprised 9,750 nt excluding the 3'-terminal poly(A) tail, with the capacity to encode a single polyprotein of 3,089 amino acids. Phylogenetic analysis with other completely sequenced potyviruses revealed that the virus in this study was most closely related to plum pox virus, with 56.3% nt identity in the genomic RNA sequence and 53.3% aa identity in the polyprotein. However, the most closely related 3'-terminal sequences were from four partially sequenced potyviruses infecting plants of the family Apiaceae (67.7-75.3% nt identity and 73.8-76.7% aa identity in their coat protein cistrons), especially Angelica virus Y. These results suggest that this virus isolate should be designated a member of a new species in the genus Potyvirus, which is tentatively named Panax virus Y (PanVY).
一种导致中草药三七（Panax notoginseng）出现畸形和花叶症状的马铃薯 Y 病毒（Potyviridae 科）从中国云南省分离得到，对一个分离株的完整核苷酸序列和另外两个分离株的部分序列进行了测定。病毒 RNA 基因组长 9750 个核苷酸，不包括 3'端聚（A）尾，能够编码一个由 3089 个氨基酸组成的单一多聚蛋白。与其他完全测序的马铃薯 Y 病毒进行的系统进化分析表明，本研究中的病毒与李痘病毒（Plum pox virus）最为密切相关，基因组 RNA 序列的核苷酸同一性为 56.3%，多聚蛋白的氨基酸同一性为 53.3%。然而，最密切相关的 3'末端序列来自四个感染伞形科植物的部分测序的马铃薯 Y 病毒（其外壳蛋白基因座的核苷酸同一性为 67.7-75.3%，氨基酸同一性为 73.8-76.7%），特别是当归病毒 Y。这些结果表明，该病毒分离株应被指定为马铃薯 Y 病毒属的一个新种成员，暂命名为三七病毒 Y（PanVY）。
